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Action Needed To Realise The Potential Of New Genetic Studies
New studies that analyse genetic differences across thousands of human genomes
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New Alternatives For Bone Imaging Could Be On The Horizon
On June 4, the U.S. Centers for Medicare & Medicaid Services (CMS) announced that it is considering a pathway for coverage of Sodium Fluoride (NaF-18) for PET bone imaging as an alternative to Technetium-99m imaging. Currently, Tc-99m bone imaging is one of the more commonly performed procedures using this radioisotope. Technetium-99m is in scant supply because of ongoing production outages, resulting in serious delays in patient imaging studies for many medical problems, including oncologic, cardiac and neurologic conditions.
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ChIP-Seq, Drosophila Targeted Mutagenesis Featured In Cold Spring Harbor Protocols
High-throughput whole-genome analysis is becoming a standard laboratory approach for investigating cellular processes. Next-generation sequencing is replacing microarrays as the technique of choice for genome-scale analysis, because it offers advantages in both sensitivity and scale. The June issue of Cold Spring Harbor Protocols features "Native Chromatin Preparation and Illumina/Solexa Library Construction" from Keji Zhao and colleagues at the National Heart, Lung and Blood Institute. The article describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using native chromatin and the Solexa/Illumina Genome Analyzer. Step-by-step instructions are given for purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP) and construction of a library for sequencing. The article is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5237).
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U.S. Must Do More To Address Maternal Mortality In Developing Countries, Rep. Moore Writes In Opinion Piece

President Obama and first lady Michelle Obama"s visit to Africa this weekend "will send a powerful message to the world about their commitment to ensuring Africa"s continued progress," Rep. Gwen Moore (D-Wis.) writes in an opinion piece in The Hill. She continues that "for Africa to make this long-forestalled progress, a renewed promise must be made to provide highly cost-effective solutions to ensure that women are healthy before, during and after pregnancy."According to Moore, "More than 500,000 women worldwide die from pregnancy each year, and millions more endure life-threatening complications." For example, in Ghana, women"s risk of pregnancy-related death is one in 45, compared with one in 4,800 in the U.S., she writes. "In some of the world"s poorest countries, including Afghanistan, the maternal death risk is as high as one in eight," Moore adds. Access to health care is a significant part of the problem, she writes, noting that "[o]nly 40% of births worldwide take place in a health facility" and that "[s]ix of the seven countries with the highest levels of maternal mortality have less than one doctor per every 10,000 people."Moore continues that she is "encouraged" that the House Appropriations Committee recently approved increased funding for family planning and maternal and child health as part of the fiscal year 2010 Foreign Operations Appropriations bill (HR 3081). "However, more remains to be done by the United States and our partners around the world if we are truly going to fulfill the promise of the Millennium Development Goals by 2015, one of which is to reduce maternal mortality by three-quarters and achieve universal access to reproductive health," she adds. Although there has been progress in fighting HIV/AIDS and working toward other Millennium Development Goals, "[w]e must recognize the appalling lack of progress that has been made in the area of maternal mortality, child mortality and family planning as major barriers to progress on all of the other goals," according to Moore."Pregnancy, childbirth and motherhood should not be a death sentence," Moore writes. She concludes, "Improving impoverished women"s chances of survival before, during and after pregnancy is an issue of rights and social justice. It is also a sound economic and social investment, given the importance of women to the well-being of their children, families and societies" (Moore, The Hill, 7/7). Reprinted with kind permission from http://www.nationalpartnership.org. You can view the entire Daily Women"s Health Policy Report, search the archives, or sign up for email delivery here. The Daily Women"s Health Policy Report is a free service of the National Partnership for Women & Families, published by The Advisory Board Company. © 2009 The Advisory Board Company. All rights reserved.


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